Botox works by blocking the nerve signals that are sent to muscles for them to contract. When the substance is injected into specific areas, it paralyzes the targeted muscles temporarily by inhibiting the release of acetylcholine, which is a neurotransmitter responsible for muscle contraction. Such relaxation of the muscles leads to a significant reduction in the appearance of dynamic wrinkles, which are usually caused by repetitive facial movements. Botox is normally injected into areas such as the forehead, between the eyebrows, and around the eyes – the areas where it smooths out wrinkles and prevents new ones from forming. The effects of the procedure are temporary, lasting three to four months, after which the muscle activity gradually returns to normal.